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超声破裂仪

DNA片断制备

  • NGS样品库制备

  • CHIP片断制备

  • 细胞亚构造提取


联系人:文密斯
电话:0755-26410427
邮箱:equipment@dakewe.com


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金沙2015 @55网站

金沙2015 @55网站

道理:

Digital Adaptive Sonocavitation(DAS)手艺即“数字式自适应超声空化”手艺,是指经由过程压电转换器,将数字式电信号,转变为高频声波旌旗灯号,发生数百万的水分子“空穴”,在几微秒内“空穴”变大、破裂发生扭曲物理力打断生物大分子。




手艺对照:

 

1)EpiSonic™ 1100接纳浅盘式水槽,底盘超声发生器大,超声波根基呈直线往上到达样本,样本受超声机械能均一,能量丧失少,能承载1-96个样本

2)Competitor D 深桶式水槽设想,超声机械能    经由过程直线大概桶壁反射抵达样本,能量丧失多,受力均一性差,承载小样本数有限(最多12个)

3)Competitor C 高频聚焦超声手艺,接纳特制的聚焦式超声发生器,聚焦点只要一个,接纳特制的样品管,高贵并且一次只能放一个;别的霎时能量异常下,致使样品升温异常严峻


温控:

在超声破裂的历程中,对样品的温度掌握,是防备样品过热,获得下重复性、下一致性效果的要害步伐。
1)  接纳直线式超声浅盘设想,EpiSonic™ 1100每一个样品管承载的能量为0.1W(低于其他产物的承载能量)
2)接纳轮回水冷却体系,经由过程冷却装配将样品槽内过剩热量,

敏捷由中周水槽流走,同时浅盘设想比深桶设想利于热水循环
3)接纳DASTM手艺,中周水槽中的火处于缓慢湍流状况,利于热量从样品传导到外周水槽


运用:

1、为DNA测序、CHIP供应优良的文库:
EpiSonic™ 1100为DNA测序、ChIP精准供应所需巨细的片断,具有下样品得率、重复性和一致性,可主动天、按预定顺序快速处置惩罚96孔PCR板或尺度实行管。
2、可取多种NGS剖析平台兼容:
EpiSonic™ 1100能够将2000bp以下的DNA剪切成下质量的、含有下一代测序
所需的DNA文库的片断,可取多种NGS剖析平台兼容:lllumina Genome Analyzer,
lllumina Hiseq,lllumina Miseq,Roche 454 Sequencing GS FLX,Roche 454
Sequencing GS Junior,Life Technologies SOLiD System,Life Technologies Ion Torrent等
3、可取多种ChIP剖析

   

平台兼容:
EpiSonic™ 1100是一款最理想的装备,可发生用于ChIP的200-600bp的片断,可取多种ChIP剖析平台兼容:ChIP-PCR/qPCR,ChIP-chip,ChIP-Seq等。
4、精确性下、一致性好、重复性好:
DNA在差别超声连续时间内被准确剪切成差别巨细的片断;将统一细胞的差别样本并行处置惩罚后,所得到的剪切片断巨细根基同等;雷同的样品分批处置惩罚后,所得到的均匀片断巨细根基雷同 

       

                              A                                  B                                                 A                                  B
                                                 Fig.1                                                                            Fig.2

 Fig.1 Demonstration of precise DNA shearing of different sizes.3µg of placental DNA in 0.2 ml tubes were sheared with the EpiSonicTM 1100 at different sonication durations(10,15,20,45,and 62.5 min).The resulting lengths of the sheared DNA were analyzed with an Agilent Bioanalyzer 2100 [A] and an agarose gel [B].

Fig.2 Demonstration of Sample-to-Sample Consistency.2µg of placental DNA in 30µl buffer were placed in well positions B2,B6,D6,E7,F9,and G11 of a 96-well PCR plate and sheared with the EpiSonicTM 1100 for 40 cycles [A] and then repeated in a subsequent run with the exact same conditions [B].

Fig.3 Demonstration of high reproducibility. 3µg sample of placental DNA (30µl ) in the different wells of a 96-well plate was sheared with the EpiSonicTM 1100 for 40 cycles.The resulting lengths of the sheared DNA were analyzed with an Agilent Bioanalyzer 2100. Average sheared DNA size is 380.4 bps for the first run [A] and is 379.2bps for the next run [B].The two Bioanalyzer imagas were then overlapped to visualize the high reproducibility [C]. The CV% of sample to sample in the same run is 1.1 to 4.7% and is 0.3% between the different runs .Average percentage of dsDNA recovered from 10 samples is 75.2%.


装备引见:

EpiSonic™ 1100体系重要包孕三个局部:超声波发生器、冷却系统、样品台。
 

    超声波发生器                                 样品台                       轮回水冷却体系    


超声波发生器
经由过程操纵 超声波发生器掌握面板,将电信号经由过程超声波转换器转换成声波旌旗灯号,传入样品室,
破裂样品,该局部具有以下长处:
触屏掌握:简朴、直观的菜单导航
下精确度:数字式调解所有的设置
及时能量、温度监测:仪器屏幕可显现振幅、瓦特、焦耳读数及样品槽内的温度等
主动超载珍爱:监测毛病,泛起毛病时可封闭体系              以防备破坏电路
可编顺序存储器:可储存10个顺序,按屏幕的数字键便可选择、挪用响应顺序

样品台
可安排尺度0.2ml PCR管、半裙管大概96孔PCR板、1.5ml的样品管
密闭容器处置惩罚:密闭的管/孔的非打仗超声波处置惩罚,能够防备样品净化和降解 
生物安全性:出有气雾发生,不消烦琐的操纵探针,进步了安全性

轮回水冷却体系
经由过程冷却器将样品室内过剩热量,经由过程输水管敏捷由中周水槽流走


产物参数:

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